## Abstract A method is described to separate α‐ from β‐arylalanines by ligand exchange chromatography on a nickel nitrilotriacetate agarose column with UV monitoring of the effluent. Separate mixtures containing an α‐ and β‐arylalanine pair (1 mg of each) were individually loaded onto the nickel r
A rapid separation of α- and β-lipoproteins by affinity chromatography
✍ Scribed by Alexis Carpenter; William C. Purdy
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 259 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0009-9120
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✦ Synopsis
A rapid and inexpensive separation of (~-and 13-1ipoproteins has been achieved using sulfated dextran beads as an affinity chromatography medium. The separation is completed in less than 15 min and the cholesterol content of the lipoprotein fractions can then be determined in a separate procedure.
📜 SIMILAR VOLUMES
A method is presented for the visualization and localization of lipoprotein bands in density gradient ultracentrifugation. The lipoproteins are stained with Sudan black prior to uhracentrifugation. The technique is suitable for studying the lipoprotein patterns of normo-and hyperlipemic sera of both