Improved techniques for the separation of serum lipoproteins by density gradient ultracentrifugation: Visualization by prestaining and rapid separation of serum lipoproteins from small volumes of serum
✍ Scribed by Anthony H.M. Terpstra; Christopher J.H. Woodward; Francisco J. Sanchez-Muniz
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 893 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A method is presented for the visualization and localization of lipoprotein bands in density gradient ultracentrifugation. The lipoproteins are stained with Sudan black prior to uhracentrifugation. The technique is suitable for studying the lipoprotein patterns of normo-and hyperlipemic sera of both humans and experimental animals. The preparation and use of various types of density gradients are also described. By using smaller centrifuge tubes and high-speed rotors, a considerable saving in centrifugation time has been achieved. Formulae have been derived which facilitate the adjustment of the density of serum and the preparation of salt solutions of known density.