Human B cell alloantigens: separation from other membrane molecules by affinity chromatography

Rapid quantitative separation of the maj

Rapid quantitative separation of the major glycoproteins (PAS 1, 2, and 3) from other human red cell membrane proteins in a nondenaturing medium by affinity chromatography

✍ Y. Shami; S. Ship; A. Rothstein 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 449 KB

Several methods were tested for separating the major glycoproteins from other proteins extracted from human red cell membranes by the nondenaturing detergent, Triton X-100. Separation could be achieved by isoelectric focusing, but the intrinsic proteins (predominantly band 3) become irreversibly pre