## Separation of amino acids as derivatives of phenylthiohydantoin (PTEkmino acids) has been performed by paper and thin-layer chromatography, gas chromatography and high-performance liquid chromatography (HPLC). EIPLC, being a rapid and highly efficient technique, is advantageous because all PTH-
Separation of amino acids by high performance liquid chromatography
β Scribed by Bayer, Ernst.; Grom, Edgar.; Kaltenegger, Berthold.; Uhmann, Rainer.
- Book ID
- 120495348
- Publisher
- American Chemical Society
- Year
- 1976
- Tongue
- English
- Weight
- 426 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0003-2700
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All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
The separation of all common dimethylaminoazobenzenethiohydantoin (DABTH) amino acids derived from modified Edman sequencing can be achieved by using high-performance liquid chromatography. All derivatives, including DABTH-Ile and DABTH-Leu, can be readily separated in a solvent mixture of sodium ac