## Separation of amino acids as derivatives of phenylthiohydantoin (PTEkmino acids) has been performed by paper and thin-layer chromatography, gas chromatography and high-performance liquid chromatography (HPLC). EIPLC, being a rapid and highly efficient technique, is advantageous because all PTH-
Separation of Dns-amino acid amides by high-performance liquid chromatography
β Scribed by William H. Simmons; Gerhard Meisenberg
- Book ID
- 108337391
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 415 KB
- Volume
- 266
- Category
- Article
- ISSN
- 1873-3778
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All common phenyhhiohydantoin amino acids derived from sequencing are identifiable in 6 to 9 min with an isocratic method based on buffered aqueous acetonitrile, an ODS stationary phase, and programmed flow which increases during the run. With constant flow the analysis requires 16 min. The effects
A high-performance liquid chromatographic analytical system is described for the separation, identification, and quantitation of phenylthiohydantoin (PTH) derivatives of all amino acids normally encountered in proteins. The system employs reverse-phase chromatography on a PBondapak phenylalkyl stati