Sensitization of Edman amino acid derivatives using electron capture sensitive reagent, 1-trifluoro-2-amino-ethane
โ Scribed by Masaharu Kamo; Mitsuru Sano; Carl S. Jone; Akira Tsugita
- Book ID
- 105273996
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 220 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-4943
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Some L-and D-amino acids (Phe or Leu) were derivatized with a fluorogenic reagent, 4-fluoro-7-nitro-2,1,3benzoxadiazole (NBD-F) and separated on a Pirkle-type column, Sumichiral OA 2500(S) [(S)-1-naphthylglyeyl-3,5-dinitrophenylamide silica gel] with a mobile phase of 20mM ammonium acetate in methan
## Abstract The usefulness of __R__(โ)โ4โ(3โisothiocyanatopyrrolidinโ1โyl)โ7โ(__N,N__โdimethylaminosulfonyl)โ2,1,3โbenzoxadiazole [__R__(โ)โDBDโPyNCS], a fluorescent chiral tagging reagent, for the determination of racemic amines and amino acids, was studied. The reagent reacted with ฮฒโblockers sel