A method for amino acid sequence and D/L configuration identification of peptides by using fluorogenic Edman reagent 7-[(N, N-dimethylamino)sulfonyl]-2,1,3-benzoxadiazol-4-yl isothiocyanate (DBD-NCS) has been developed. This method was based on the Edman degradation principle with some modifications
✦ LIBER ✦
Development of an Efficient Amino Acid Sequencing Method Using Fluorescent Edman Reagent 7-[(N,N-Dimethylamino)sulfonyl]-2,1,3-benzoxadiazol-4-yl Isothiocyanate
✍ Scribed by Matsunaga, Hirokazu.; Santa, Tomofumi.; Hagiwara, Kenichi.; Homma, Hiroshi.; Imai, Kazuhiro.; Uzu, Sonoko.; Nakashima, Kenichiro.; Akiyama, Shuzo.
- Book ID
- 126417178
- Publisher
- American Chemical Society
- Year
- 1995
- Tongue
- English
- Weight
- 684 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0003-2700
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## Abstract The usefulness of __R__(−)‐4‐(3‐isothiocyanatopyrrolidin‐1‐yl)‐7‐(__N,N__‐dimethylaminosulfonyl)‐2,1,3‐benzoxadiazole [__R__(−)‐DBD‐PyNCS], a fluorescent chiral tagging reagent, for the determination of racemic amines and amino acids, was studied. The reagent reacted with β‐blockers sel