Sensitive determination of enantiomers of amino acids derivatized with the fluorogenic reagent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole, separated on a pirkle-type column, sumichiral OA 2500(S)
✍ Scribed by Kazuhiro Imai; Takeshi Fukushima; Sonoko Uzu
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 159 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0269-3879
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✦ Synopsis
Some L-and D-amino acids (Phe or Leu) were derivatized with a fluorogenic reagent, 4-fluoro-7-nitro-2,1,3benzoxadiazole (NBD-F) and separated on a Pirkle-type column, Sumichiral OA 2500(S) [(S)-1-naphthylglyeyl-3,5-dinitrophenylamide silica gel] with a mobile phase of 20mM ammonium acetate in methanol. The fluorometric detection was made at 530 nm with excitation at 470 nm. No racemization of the enantiomers occurred during the derivatization reaction. The separation factors (0) for NBD-L-Phe and NBD-D-Phe, and NBU-L-LXU and NBD-u-Leu, were 1.27 and 1.17. respectively. The detection limits were in the range of ca.