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Sensitive determination of N-terminal prolyl peptides by high-performance liquid chromatography with laser-induced fluorescence detection

โœ Scribed by Toshimasa Toyo'oka; Mumio Ishibashi; Tadao Terao


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
701 KB
Volume
661
Category
Article
ISSN
1873-3778

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Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co