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High-performance liquid chromatography of N-terminal tryptophan-containing peptides with precolumn fluorescence derivatization with glyoxal

✍ Scribed by Masaaki Kai; Eijiro Kojima; Yosuke Ohkura; Masatake Iwasaki

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
508 KB
Volume
653
Category
Article
ISSN
1873-3778

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## Abstract HPLC was coupled with sequential injection (SI) for simultaneous analyses of some heavy metals, including Co(II), Ni(II), Cu(II), and Fe(II). 2‐(5‐Nitro‐2‐pyridylazo)‐5‐[__N__‐propyl‐__N__‐(3‐sulfopropyl)amino]phenol (nitro‐PAPS) was employed as a derivatizing reagent for sensitive spec