A liquid chromatographic (LC) method to assay homocysteine in plasma at micro-molar levels is described. The procedure consists of protein precipitation with trichloroacetic acid and a centrifugation step. The supernatant is then derivatized with a thiol-specific fluorochromophore and LC assayed wit
Determination of plasma homocysteine by high-performance liquid chromatography with fluorescence detection
β Scribed by Donald W. Jacobsen; Vytenis J. Gatautis; Ralph Green
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 787 KB
- Volume
- 178
- Category
- Article
- ISSN
- 0003-2697
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## Abstract We here describe an ionβexchange highβperformance liquid chromatography technique with electrochemical detection for rapid quantification of glutathione, homocysteine, cysteinylglycine, and methionine. The analytical validation of the technique showed withinβassay and betweenβassay coef