Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co
β¦ LIBER β¦
Quantitative Determination of Monosaccharides in Glycoproteins by High-Performance Liquid Chromatography with Highly Sensitive Fluorescence Detection
β Scribed by K.R. Anumula
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 719 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0003-2697
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