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Determination of Monosaccharides in Glycoproteins by Reverse-Phase High-Performance Liquid Chromatography

โœ Scribed by H. Kwon; J. Kim

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
517 KB
Volume
215
Category
Article
ISSN
0003-2697

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Monosaccharide determination of glycocon
Monosaccharide determination of glycoconjugates by reverse-phase high-performance liquid chromatography of their phenylthiocarbamyl derivatives
โœ Mary Jane Spiro; Robert G. Spiro ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 687 KB

A method for the determination of neutral sugars and hexosamines present in glycoconjugates by reverse-phase high-performance liquid chromatography (HPLC) of their phenylthiocarbamyl (PTC) derivatives has been developed. After acid hydrolysis, neutral sugars are converted to glycamines by reaction w