✦ LIBER ✦

Sensitive assay for cellulase and dextranase

✍ Scribed by Jung San Huang; Jordan Tang

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 462 KB
Volume: 73
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90182-2

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✦ Synopsis

Sensitive, rapid, and quantitative methods have been devised for the assay of cellulases and dextranases through the synthesis of two chemically modified carboxymethyl cellulose substrates. One contains a trinitrophenyl group as chromophore. The other contains a fluorescent fluorescamine group. The soluble hydrolytic products in the filtrate released from the substrates by cellulase are thus monitored either spectrophotometrically (for trinitrophenyl group) or spectrofluorometrically (for fluorescamine). The same principle has been applied to the determination of dextranases by utilizing chemically modified Sephadex G-200 containing either group as described above for carboxymethyl cellulose. The methods are sensitive to about 5 pg of enzyme for trinitrophenylcontaining substrates, while the use of fluorescamine-containing substrates is about tenfold more sensitive.

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