𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A rapid and sensitive assay for arginase

✍ Scribed by Urs T. Rüegg; Anthony S. Russell

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
516 KB
Volume
102
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

An assay for arginase is described that uses L-[guanido-'"Clarginine as substrate. Unhydrolyzed arginine is removed in a batch procedure with sulfonate resin and the ['"Clurea product is determined quantitatively in the resin supernatant. The assay requires 5 min and is performed in one tube. The sensitivity is approximately 0.1 munits of arginase. Arginase activities in fetal calf serum and in murine macrophage extract have been determined and the bovine liver enzyme has been used as a reference.

📜 SIMILAR VOLUMES

A radiometric assay for arginase
A radiometric assay for arginase
✍ Piergiorgio Righetti; Luigi De Luca; George Wolf 📂 Article 📅 1968 🏛 Elsevier Science 🌐 English ⚖ 316 KB
A rapid sensitive collagenase assay
A rapid sensitive collagenase assay
✍ M.T. Gisslow; B.C. McBride 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 448 KB

A rapid, sensitive collagenase assay has been developed using 14C-acetylated collagen as a substrate. Acid-soluble calfskin collagen was labeled with [1-14C]acetic anhydride at pH 8. The acetylated collagen had a specific activity of 6.25 × l0 ~ dpm/mg protein. Collagen was not denatured as evidence

A rapid and sensitive assay method for p
A rapid and sensitive assay method for protein kinase
✍ Kuo-Ping Huang; J.C. Robinson 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 421 KB

A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein

A rapid, sensitive assay for starch phos
A rapid, sensitive assay for starch phosphorylase and ADPglucose pyrophosphorylase
✍ Derek A. McCracken; William M. Rutherford 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 212 KB

Starch phosphorylase (EC 2.4.1.1) and ADPglucose pyrophosphorylase (EC 2.7.7.27) activities can be measured very accurately and quickly using a recording pH meter. Activities less than 0.28 nmolimin are readily measured.