A new, rapid and sensitive assay of ADPglucose pyrophosphorylase using the luciferaseluciferin system to quantify the reaction product, ATP, is described. The total assay time per sample is about 10 min. The sensitivity ranges from picomoles to nanomoles, and the crude enzyme extract from individual
A rapid, sensitive assay for starch phosphorylase and ADPglucose pyrophosphorylase
โ Scribed by Derek A. McCracken; William M. Rutherford
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 212 KB
- Volume
- 101
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
Starch phosphorylase (EC 2.4.1.1) and ADPglucose pyrophosphorylase (EC 2.7.7.27) activities can be measured very accurately and quickly using a recording pH meter. Activities less than 0.28 nmolimin are readily measured.
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