๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A rapid, sensitive assay for starch phosphorylase and ADPglucose pyrophosphorylase

โœ Scribed by Derek A. McCracken; William M. Rutherford


Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
212 KB
Volume
101
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis


Starch phosphorylase (EC 2.4.1.1) and ADPglucose pyrophosphorylase (EC 2.7.7.27) activities can be measured very accurately and quickly using a recording pH meter. Activities less than 0.28 nmolimin are readily measured.


๐Ÿ“œ SIMILAR VOLUMES


A rapid and sensitive assay of ADPglucos
โœ Te May Ching ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 310 KB

A new, rapid and sensitive assay of ADPglucose pyrophosphorylase using the luciferaseluciferin system to quantify the reaction product, ATP, is described. The total assay time per sample is about 10 min. The sensitivity ranges from picomoles to nanomoles, and the crude enzyme extract from individual

A rapid and sensitive assay method for p
โœ Kuo-Ping Huang; J.C. Robinson ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 421 KB

A simplified assay method is described for the determination of protein kinase activity. Enzymatic activity is followed by measuring the incorporation of 32P from the terminal phosphoryl group of nucleoside triphosphates into protein substrate. Separation of the resulting 32P-labeled phosphoprotein

A rapid and sensitive liquid chromatogra
โœ S. Nesnow; C. Heidelberger ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 300 KB

A rapid method for the assay of epoxide hydrase activity is described. 3-Methylcholanthrene-11.1 Z-oxide is employed as substrate and high speed liquid chromatography is used to separate and quantitate tram-11. I ?-dihydro-I 1.12dihydroxy-3-methylcholanthrene (product) formation. The determination o

A rapid and sensitive fluorimetric assay
โœ Donald G. Gardiner ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 481 KB

A rapid and sensitive fluorimetric assay for inosine and adenosine is described in which either or both metabolites are enzymically converted to hypoxanthine, which is then estimated by the rate of appearance of hydrogen peroxide after the addition of xanthine oxidase. The method has been used to as