Starch phosphorylase (EC 2.4.1.1) and ADPglucose pyrophosphorylase (EC 2.7.7.27) activities can be measured very accurately and quickly using a recording pH meter. Activities less than 0.28 nmolimin are readily measured.
A rapid and sensitive assay of ADPglucose pyrophosphorylase using luciferase
โ Scribed by Te May Ching
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 310 KB
- Volume
- 111
- Category
- Article
- ISSN
- 0003-2697
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โฆ Synopsis
A new, rapid and sensitive assay of ADPglucose pyrophosphorylase using the luciferaseluciferin system to quantify the reaction product, ATP, is described. The total assay time per sample is about 10 min. The sensitivity ranges from picomoles to nanomoles, and the crude enzyme extract from individual seeds of cereals can be assayed.
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