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A rapid and sensitive assay of ADPglucose pyrophosphorylase using luciferase

โœ Scribed by Te May Ching


Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
310 KB
Volume
111
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


A new, rapid and sensitive assay of ADPglucose pyrophosphorylase using the luciferaseluciferin system to quantify the reaction product, ATP, is described. The total assay time per sample is about 10 min. The sensitivity ranges from picomoles to nanomoles, and the crude enzyme extract from individual seeds of cereals can be assayed.


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