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A rapid and sensitive lipase assay using triethylaminoethyl cellulose columns

โœ Scribed by Franklin J. Zieve

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
336 KB
Volume
51
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A procedure has been developed for the separation of labeled fatty acids from tri-, di-, and monoglycerides using small disposable columns of TEAE-cellulose.

This procedure is used as the basis of a lipase assay which is rapid, sensitive and unaffected by wide variations in the composition of the reaction mixtures. 0.5 nmole ['4C101eic acid can be debected, and the entire procedure requires less than 3 min.

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