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A sensitive and simple assay of starch synthase activity with pyruvate kinase and luciferase

✍ Scribed by Te May Ching

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
408 KB
Volume
122
Category
Article
ISSN
0003-2697

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✦ Synopsis

This paper describes a new, sensitive, simple, rapid, and inexpensive assay of starch synthase using pyruvate kinase and phosphoenolpyruvate to convert the reaction product, ADP, to ATP, which is then quantified by use of the luciferin-luciferase system. The assay procedure covers the picomole to nanomole range, involves one-step incubation, takes less than 20 mitt, and does not involve use of a radioactive tracer. Partially purified soluble and starch granular enzymes of cereals can readily be assayed in small quantities of material.

' Oregon Agricultural Experiment Station Technical Paper No. 6187.

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