𝔖 Bobbio Scriptorium
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A sensitive assay for allantoin

✍ Scribed by John Abraham; Fiorindo A. Simeone; Robert W. Hopkins

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
214 KB
Volume
70
Category
Article
ISSN
0003-2697

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✦ Synopsis

Allantoin is separated by thin-layer chromatography (TLC) and sprayed with an acidic solution ofp-dimethylaminobenzaldehyde. The yellow color produced is read in a densitometer and compared with that of a standard. The lower limit of quantitation is 0.1 ~g per spot (0.5 mg/100 ml). The method can be utilized for the estimation of allantoin in serum, lymph, and urine.

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