𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A sensitive assay for histone methyltransferase

✍ Scribed by James C. Wallwork; Catherine T. Lee; John A. Duerre

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
448 KB
Volume
84
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

A method is described for the assay of histone methyltransferase using soluble histones as substrate. The precipitation of the methylated protein on chromatography paper allows for greater sensitivity and more rapid sample processing than have previously been reported. After incubation of the enzyme in the presence of radioisotopically labeled S-adenosyl-L-methionine and soluble rat brain histone, the residual S-adenosyk-methionine is removed by extensive washing in 1.1 M trichloroacetic acid. The amount of methyl groups incorporated into histones is measured by liquid scintillation counting. This procedure can probably be used to assay other protein methylases. A comparison is made between this assay and one using chromosomal bound histones as substrates.

πŸ“œ SIMILAR VOLUMES

A more sensitive assay for histone deace
A more sensitive assay for histone deacetylase
✍ Jaap H. Waterborg; Harry R. Matthews πŸ“‚ Article πŸ“… 1982 πŸ› Elsevier Science 🌐 English βš– 425 KB

The new assay uses as substrate a peptide derived from the amino terminal domain of calf histone H4. The peptide contains all the lysines that are acetylated in H4 in vivo and these lysines are specifically labeled in vitro with acetic anhydride to a high specific activity. This substrate allows his

A nonisotopic assay for acetylserotonin
A nonisotopic assay for acetylserotonin methyltransferase
✍ Osamu Suzuki; Kunio Yagi πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 333 KB

A nonisotopic assay for acetylserotonin methyltransferase (ASMT) was devised. Melatonin, the product of the enzyme reaction, is measured fluorometrically after its reaction with o-phthaldialdehyde (OPT). The reaction of melatonin with OPT is carried out in 1 N HCI to suppress the reaction of N-acety

A sensitive assay for allantoin
A sensitive assay for allantoin
✍ John Abraham; Fiorindo A. Simeone; Robert W. Hopkins πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 214 KB

Allantoin is separated by thin-layer chromatography (TLC) and sprayed with an acidic solution ofp-dimethylaminobenzaldehyde. The yellow color produced is read in a densitometer and compared with that of a standard. The lower limit of quantitation is 0.1 ~g per spot (0.5 mg/100 ml). The method can be