✦ LIBER ✦

A more sensitive assay for histone deacetylase

✍ Scribed by Jaap H. Waterborg; Harry R. Matthews

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 425 KB
Volume: 122
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90288-3

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✦ Synopsis

The new assay uses as substrate a peptide derived from the amino terminal domain of calf histone H4. The peptide contains all the lysines that are acetylated in H4 in vivo and these lysines are specifically labeled in vitro with acetic anhydride to a high specific activity. This substrate allows histone deacetylase activity to be measured economically and with high sensitivity either with pure enzyme or with crude extracts.

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