𝔖 Bobbio Scriptorium
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A kinetic assay for cellulases

✍ Scribed by D.F. Day; W.E. Workman

Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
217 KB
Volume
126
Category
Article
ISSN
0003-2697

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✦ Synopsis

Research on the mechanism of action of cellulases has been hampered by the lack of a rapid, continuous, or kinetic assay. A linked assay system that uses glucose oxidase and horseradish peroxidase has been coupled with @-glucosidase to yield an assay system that can be used for kinetic assays for cellobiase-producing enzymes as well as a measure of cellobiose degradation by (5glucosidases. This assay shows a 20-fold increase in sensitivity over the traditional reducing sugar assay.

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