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A new kinetic assay method for quinone-reducing enzymes

โœ Scribed by E.P. Titovets; G.G. Petrovsky

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
446 KB
Volume
81
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A new kinetic method is described for the assay of quinone-reducing enzymes in various biological materials. It is based on polarographic determination of oxygen uptake in spontaneous oxidation of the diphenol formed as a result of 4-anilino-5-methoxybenzoquinone-1 ,2 (AMOBQ) enzymic reduction. The stoichiometry of the reducing equivalent transfer in the reaction sequence from NAD(P)H to oxygen has been analyzed. Data are presented on quinone-reducing activity distributions in different tissues.

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