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A kinetic isotope dilution assay for glycerol

โœ Scribed by John D. Bell; Iain L.O. Buxton; Laurence L. Brunton

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
329 KB
Volume
139
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Using glycerol kinase and [3H]glycerol, a kinetic isotope dilution assay for glycerol has been developed. Reactant and product are separated by stepwise elution from QAE-Sephadex. This assay is sensitive to as little as 100 pmol of glycerol, avoids numerous drawbacks of the traditional fluorescent assay, and readily detects glycerol production by fewer than 10(5) cardiomyocytes.

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