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Selection for temperature-sensitive mutants of diploid and tetraploid mammalian cells

✍ Scribed by Michael W. McBurney; Gordon F. Whitmore

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
429 KB
Volume
83
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Mammalian cell populations may be enriched for temperature‐sensitive (ts) mutants by the tritiated thymidine (^3^H‐TdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the near‐diploid Chinese hamster ovary (CHO) cell line and on a line made “tetraploid” by Colcemid treatment. Clones of ts mutants were obtained from the diploid line, but in spite of repeated attempts, no ts mutants were isolated from the tetroploid line. The phenotypes of the new diploid ts mutants recovered were consistent with the particular selection regime employed.

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