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Isolation and cell cycle analysis of temperature-sensitive mutants from chinese hamster cells

✍ Scribed by Jose A. Melero

Publisher
John Wiley and Sons
Year
1979
Tongue
English
Weight
905 KB
Volume
98
Category
Article
ISSN
0021-9541

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✦ Synopsis

Mutants temperature-sensitive for growth have been isolated from the established line of Chinese hamster fibroblasts Wg1A. These mutants, together with the ones previously isolated by Roscoe et al. ('73), have been characterized with regard to their cell cycle properties. Most of them become arrested in the G1 phase of the cell cycle when incubated under restrictive conditions. By performing temperature shift experiments with synchronous cultures, the execution steps of most of the mutated functions have been located within the second half of the G1 phase.

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