𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Isolation and preliminary characterization of bleomycin-resistant mutants from Chinese hamster ovary cells

✍ Scribed by Shin-Ichi Akiyama; Michihiko Kuwano

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
511 KB
Volume
107
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Stable mutants resistant to an anticancer antibiotic, bleomycin‐A2, were selected in Chinese hamster ovary (CHO) cell either spontaneously or after ethylmethane sulfonate mutagenesis. Fluctuation analysis showed that bleomycin resistance occurs in CHO at a rate of 6.50–6.58 × 10^−7^ mutations per cell per generation. Bleomycin‐A2‐resistant cell lines exhibited increased resistance to bleomycin analogs—bleomycin‐A5, ‐B2, ‐B4, and pepleomycin. Colchicine, mitomycin C, and ultraviolet light irradiation inhibited colony formation equally in CHO cells and in bleomycin‐resistant mutants. Cell‐cell hybridization tests showed that bleomycin‐resistance behaves as a dominant trait. Bleomycin‐in‐activating activity in the mutant cell extracts was three to fourfold higher than that in extracts of the parental CHO cell.

📜 SIMILAR VOLUMES

Isolation and characterization of tunica
Isolation and characterization of tunicamycin resistant mutants from Chinese Hamster ovary cells
✍ Tadashi Sudo; Kazukiyo Onodera 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 671 KB

## Abstract Stable clones selected for resistance to tunicamycin (TM) have been isolated from Chinese Hamster Ovary (CHO) cells. The TM^R^ phenotype is stable for more than nine months in the absence of the drug. The morphology of TM^R^ mutant varies from epitheloid to abnormally elongate. The muta

Taxol resistant mutants of Chinese hamst
Taxol resistant mutants of Chinese hamster ovary cells: Genetic biochemical, and cross-resistance studies
✍ Radhey S. Gupta 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 English ⚖ 706 KB

The effects of the microtubule inhibitor taxol on the growth and viability of Chinese hamster ovary (CHO) cells have been examined. Stable mutants which are between seven to 11-fold more resistant to taxol have been selected in a single step from ethyl methanesulfonate-mutagenized CHO cells. The two

Mutants of Chinese hamster cells resista
Mutants of Chinese hamster cells resistant to adenosine
✍ Michael W. McBurney; Gordon F. Whitmore 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 English ⚖ 949 KB

## Abstract Chinese hamster cells do not grow in medium containing high concentrations of adenosine because pyrimidine biosynthesis is inhibited. Adenosine metabolism was examined in two mutant cell lines isolated on the basis of resistance to adenosine. One line was deficient in adenosine kinase s

Ribonucleotide reductase from wild type
Ribonucleotide reductase from wild type and hydroxyurea-resistant chinese hamster ovary cells
✍ William H. Lewis; Jim A. Wright 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 842 KB

## Abstract The kinetic properties of partially purified ribonucleotide reductase from Chinese hamster ovary cells have been investigated. Double reciprocal plots of velocity against substrate concentration were found to be linear for three the substrates tested, and yielded apparent Km values of 0

Drug-resistant mutants of chinese hamste
Drug-resistant mutants of chinese hamster ovary cells possess an altered cell surface carbohydrate component
✍ Juliano, Rudy ;Ling, Victor ;Graves, James 📂 Article 📅 1976 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 291 KB

## Abstract Surface label experiments using the galactose oxidase‐[^3^ H] ‐borohydride technique reveal that cells from drug‐resistant Chinese hamster ovary clones possess a surface carbohydrate component of apparent molecular weight 165,000 which is absent from wild‐type cells. The component may a