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Mutants of Chinese hamster cells resistant to adenosine

✍ Scribed by Michael W. McBurney; Gordon F. Whitmore

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 949 KB
Volume: 85
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040850110

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Chinese hamster cells do not grow in medium containing high concentrations of adenosine because pyrimidine biosynthesis is inhibited. Adenosine metabolism was examined in two mutant cell lines isolated on the basis of resistance to adenosine. One line was deficient in adenosine kinase suggesting that high intracellular AMP concentrations may block pyrimidine synthesis indirectly in wild type cells by inhibiting PRPP synthetase. Although no enzymatic defect could be identified in the other cell line, these cells inefficiently utilize adenosine supplied in the medium.

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