✦ LIBER ✦

Isolation and characterization of tunicamycin resistant mutants from Chinese Hamster ovary cells

✍ Scribed by Tadashi Sudo; Kazukiyo Onodera

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 671 KB
Volume: 101
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041010117

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Stable clones selected for resistance to tunicamycin (TM) have been isolated from Chinese Hamster Ovary (CHO) cells. The TM^R^ phenotype is stable for more than nine months in the absence of the drug. The morphology of TM^R^ mutant varies from epitheloid to abnormally elongate. The mutants do not display cross‐resistance for ConA but are slightly cross‐resistant to PHA. Biochemically labeled membrane proteins and glycoprotein of Vesicular stomatitis virus (VSV) grown in the TM^R^ mutants revealed that the incorporation of radioactive glucosamine was markedly reduced in the mutants. The results indicate that TM^R^ cells are a novel type of membrane mutant.

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