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Changes in RNA polymerase II in a cell cycle-specific temperature-sensitive mutant of hamster cells

✍ Scribed by Mara Rossini; Susan Baserga; C. H. Huang; C. James Ingles; Renato Baserga

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
657 KB
Volume
103
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

tsAF8 cells are a temperature‐sensitive mutant of BHK cells that arrest at the nonpermissive temperature in the G~1~ phase of the cell cycle. The activity of solubilized RNA polymerase II and its ability to bind [^3^H]‐γ‐amanitin decrease in tsAF8 cells at 40.6°, with a half‐life of ∼ 10 hr. No appreciable changes occur in these two parameters in tsAF8 cells at 34° or in BHK cells at either 34° or 40.6°. Protein synthesis is not appreciably affected for at least 24 hr after tsAF8 cells are shifted to 40.6°. These results indicate that in tsAF8 cells at the nonpermissive temperature, there is a defect in either the synthesis, the assembly, or the stability of RNA polymerase II, and that the loss of RNA polymerase II molecules is not due to widespread cellular damage.

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