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Defective RNA polymerase II in the G1 specific temperature sensitive hamster cell mutant TsAF8

✍ Scribed by Michael Shales; John Bergsagel; C. James Ingles

Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
512 KB
Volume
105
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

TsAF8 is a temperature‐sensitive (TS) mutant of BHK21 cells that arrests at nonpermissive temperatures in the mid‐G~1~ phase of the cell cycle. TsAma^R^‐1 is a TS for growth mutant of CHO cells with a Ts‐ and α‐amanitin‐resistant (Ama^R^) RNA polymerase II activity. Hybrid TsAma^R^‐1 x TsAF8 cell lines were constructed at permissive temperatures. Such hybrid cells did not grow at nonpermissive temperatures; the two TS mutations did not complement. Two different Ama^R^ derivatives of TsAF8 were isolated. Each contained only Ama^R^ polymerase II activity, indicating that this RNA polymerase II gene locus in TsAF8 is functionally hemizygous, as would be expected for a locus in which the recessive TsAF8 mutation had occurred. One of these Ama^R^ isolates of TsAF8 had a partially reverted TS^+^ phenotype. Taken together these results suggest that the TS mutation in TsAF8 is in RNA polymerase II.

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