## Abstract Mammalian cell populations may be enriched for temperatureโsensitive (__ts__) mutants by the tritiated thymidine (^3^HโTdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the nearโdiploid Chinese hamster ovary (CHO) cell line and on a line made โtetraploid
Application of temperature-sensitive mutants for single-cell protein production
โ Scribed by Yuichiro Miyasaka; Chokyun Rha; Anthony J. Sinskey
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 571 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3592
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## Abstract A temperatureโsensitive mutant K12 isolated from an established line of Chinese hamster fibroblasts has been identified as a cellโcycle mutant blocked in G1 (Roscoe et al., '73a, b). While characterizing the phenotype of K12 in more detail, we observed that K12 cells are highly sensitiv