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Isolation of temperature sensitive mammalian cells by selective detachment

✍ Scribed by D. H. Roscoe; Moira Read; Hildred Robinson

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
563 KB
Volume
82
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Temperature sensitive cells have been isclated from Syrian and Chinese hamster cells using a method based on selective detachment from a glass substrate. The Syrian hamster isolates occurred at a high frequency (about 1 in 10^3^) and reverted rapidly; polyoma virus transformation conferred on cells the ability to grow, perhaps abnormally, in agar suspension. A slightly modified isolation technique was applied to Chinese hamster cultures and resulted in the isolation of at least one mutant (from a starting population of 5 × 10^8^ cells) with a spontaneous reversion rate of less than one in 6 × 10^7^. Treatment of the mutant with ethyl methane sulphonate induced reversion. It was concluded that selective detachment provided a useful method for the isolation of conditional lethal mutants of mammalian cells.

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