Selected reaction monitoring during gas chromatography/mass spectrometry of eicosanoids

✍ Scribed by Helen Hughes; Jean Nowlin; Simon J. Gaskell; Victor C. Parr

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 402 KB
• Volume: 16
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200160180

✦ Synopsis

Gas

chromatography/electron capture negative ion mass spectrometry of eicosanoids, as pentafluorobenzyl (PFB) ester, methyl oxime (where applicable), trimethylsilyl (TMS) ether derivatives, is reported using a double-focusing instrument of trisector configuration. Sub-picogram detection limits were observed for prostaglandins El, E, and F,, during selected ion monitoring (SIM) of [M -PFBJ-ions. Selected reaction monitoring (SRM) of [M -PFBl-+ [M -PFB -TMSOHI-, occurring in the first field-free region, was of modest sensitivity, reflecting the stability of the (M -PFBJ-ions. The leukotriene B, (LTB,) derivative was successfully analyzed by SIM at the low-picogram level. In this instance, the fragmentation [M -PFBl-+ [M -PFB -TMSOH] occurred in high yield in the first field-free region. The advantageous improvement in selectivity of detection that may be achieved with SRM was evident during the analysis of a serum extract for LTB, .