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Sedimentation equilibrium of DNA samples heterogeneous in density

✍ Scribed by Carl W. Schmid; John E. Hearst

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
382 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

The problem of determining the molecular weight of DNA samples by sedimentation equilibrium in a buoyant‐density gradient is considered for the case of DNA samples with density heterogeneity. By determining apparent molecular weights in two or more buoyant mediums, quantitative measure of the amount of density heterogeneity can be determined. This method may be employed to determine both the true molecular weight and the extent of base composition heterogeneity.

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