COMMUNICATlON TO THE EDITORS ## Molecular Weights from Equilibrium Sedimentation in a Deneity Gradient* A mixed solvent, containing a light component 1 and a heavy component 3, generates a density gradient when sedimented at high speeds in an ultracentrifuge. If initial density conditions are pro
Molecular weight of DNA from sedimentation equilibrium in CsCl density gradient
β Scribed by Ezra Daniel
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1969
- Tongue
- English
- Weight
- 118 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0006-3525
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π SIMILAR VOLUMES
Wherever -\Ip-, -Urn-, aP-, rp-, \_ I f : -, ii-is printed, replace with JID-,
## Abstract The problem of determining the molecular weight of DNA samples by sedimentation equilibrium in a buoyantβdensity gradient is considered for the case of DNA samples with density heterogeneity. By determining apparent molecular weights in two or more buoyant mediums, quantitative measure
The sedimentation properties of a P. mimbilis DNA sample have been investigated at different concentrations and rotor speeds. Pronounced speed effects occurred at high angular velocities. The s&w value evaluated from low-speed experiments amounts to 61 S., indicating a mean molecular weight of 105 m