Molecular weights from equilibrium sedimentation in a density gradient

✍ Scribed by Henryk Eisenberg

• Publisher: Wiley (John Wiley & Sons)
• Year: 1967
• Tongue: English
• Weight: 147 KB
• Volume: 5
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1967.360050709

✦ Synopsis

COMMUNICATlON TO THE EDITORS

Molecular Weights from Equilibrium Sedimentation in a Deneity Gradient*

A mixed solvent, containing a light component 1 and a heavy component 3, generates a density gradient when sedimented at high speeds in an ultracentrifuge. If initial density conditions are properly chosen, then a macromolecular component 2, present in small amount, forms a "Gaussian" band, at equilibrium in the centrifuge cell; the halfwidth of the band is, all other things being equal, inversely proportional to the square root of the molecular weight Ma of the macromolecular component. This method was 6rst * presented at the Conference on the Characterization of Macromolecular St.ructure, National Reeearch Council, Warrenton, Virginis, April 5-7,1967. d In cf/dr = (~C/RT)M~(bp/bci),,r (ap/bci),, = (1 -A ~) -&(Ihpo) and York, 1964, Chap. 6.7. (1961).