Concentration dependence in three-component systems in sedimentation equilibrium in buoyant density gradients
β Scribed by Pak H. Poon; Verne N. Schumaker
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1971
- Tongue
- English
- Weight
- 277 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0006-3525
No coin nor oath required. For personal study only.
β¦ Synopsis
The data on the band widths and band shapes of several DNA's a t various concentrations in sedimentation equilibrium in a CsCl density gradient have recently become available. In the present report, these literature data are treated in the following manner:
( 1 ) based on a theory of isotope-substitution, calculations are made of the molecular weights at infinite dilution, and (2) to explain the concentration dependence of band widths and band shapes, a theory of charge and hydration is put forth, and it is shown that by retaining the terms involving the charge of the macromolecule, it is possible to sccoiuit for most of the concentration dependence.
π SIMILAR VOLUMES
## Abstract The theory for the approach to equilibrium in a buoyantβdensity gradient is experimentally examined for the case of DNA in CsCl.
COMMUNICATlON TO THE EDITORS ## Molecular Weights from Equilibrium Sedimentation in a Deneity Gradient* A mixed solvent, containing a light component 1 and a heavy component 3, generates a density gradient when sedimented at high speeds in an ultracentrifuge. If initial density conditions are pro
Wherever -\Ip-, -Urn-, aP-, rp-, \_ I f : -, ii-is printed, replace with JID-,