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Concentration dependence in three-component systems in sedimentation equilibrium in buoyant density gradients

✍ Scribed by Pak H. Poon; Verne N. Schumaker


Publisher
Wiley (John Wiley & Sons)
Year
1971
Tongue
English
Weight
277 KB
Volume
10
Category
Article
ISSN
0006-3525

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✦ Synopsis


The data on the band widths and band shapes of several DNA's a t various concentrations in sedimentation equilibrium in a CsCl density gradient have recently become available. In the present report, these literature data are treated in the following manner:

( 1 ) based on a theory of isotope-substitution, calculations are made of the molecular weights at infinite dilution, and (2) to explain the concentration dependence of band widths and band shapes, a theory of charge and hydration is put forth, and it is shown that by retaining the terms involving the charge of the macromolecule, it is possible to sccoiuit for most of the concentration dependence.


πŸ“œ SIMILAR VOLUMES


The approach to equilibrium in a buoyant
✍ Carl W. Schmid; John E. Hearst πŸ“‚ Article πŸ“… 1972 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 301 KB

## Abstract The theory for the approach to equilibrium in a buoyant‐density gradient is experimentally examined for the case of DNA in CsCl.

Molecular weights from equilibrium sedim
✍ Henryk Eisenberg πŸ“‚ Article πŸ“… 1967 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 147 KB

COMMUNICATlON TO THE EDITORS ## Molecular Weights from Equilibrium Sedimentation in a Deneity Gradient* A mixed solvent, containing a light component 1 and a heavy component 3, generates a density gradient when sedimented at high speeds in an ultracentrifuge. If initial density conditions are pro