Salt-induced protein precipitation in aqueous solution: Single and binary protein systems
β Scribed by Sang Gon Kim; Young Chan Bae
- Publisher
- The Polymer Society of Korea
- Year
- 2003
- Tongue
- English
- Weight
- 538 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1598-5032
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Protein-protein and protein-salt interactions have been obtained for ovalbumin in solutions of ammonium sulfate and for lysozyme in solutions of ammonium sulfate, sodium chloride, potassium isothiocyanate, and potassium chloride. The two-body interactions between ovalbumin molecules in concentrated
An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG ph