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Dissociation and isolation of chromatin proteins in salt solutions by an aqueous two-phase system

✍ Scribed by Arúnas A. Gineitis; Sigita P. Sučiliene; Vasant P. Shanbhag

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
649 KB
Volume
139
Category
Article
ISSN
0003-2697

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✦ Synopsis

An aqueous two-phase system containing 7% Dextran T 500-5% polyethylene glycol (PEG) 6000 has been adopted for rapid selective stepwise extractions of high-mobility-group proteins and histones from both isolated chromatin and intact nuclei of calf thymus. After the dissociated proteins in the PEG phase were precipitated with 20% trichloroacetic acid at 4 degrees C, proteins were recovered from this phase by solubilization of PEG with acidified acetone at room temperature. This method allows preparation of nuclei depleted of histone H1.

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