Use of the thermodynamic principles of aqueous two-phase extraction (ATPE) to drive protein into a crosslinked gel is developed as a protein isolation and separation technique, and as a protein loading technique for drug delivery applications. A PEG/dextran gel system was chosen as a model system be
β¦ LIBER β¦
Extraction of Proteins from Biological Fluids by Use of an Ionic Liquid/Aqueous Two-Phase System
β Scribed by Zhuo Du; Yong-Liang Yu; Jian-Hua Wang
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 175 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0947-6539
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## Abstract The influence of four variables, specifically PEG molar mass (400, 1,000, and 8,000 g/mol), concentrations of PEG and phosphate salts (15, 20, and 25% for both), and agitation intensity (110, 150, and 200 rpm), on clavulanic acid (CA) extraction by extractive fermentation with PEG/phosp
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