Use of the thermodynamic principles of aqueous two-phase extraction (ATPE) to drive protein into a crosslinked gel is developed as a protein isolation and separation technique, and as a protein loading technique for drug delivery applications. A PEG/dextran gel system was chosen as a model system be
โฆ LIBER โฆ
Recovery and separation of cell lysate proteins using hydrogels guided by aqueous two-phase extraction principles
โ Scribed by Christopher S. Putka; Stevin H. Gehrke; Mike Willis; David Stafford; John Bryant
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 97 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Protein sorption and recovery by hydroge
โ
Stevin H. Gehrke; Nitin R. Vaid; James F. McBride
๐
Article
๐
1998
๐
John Wiley and Sons
๐
English
โ 115 KB
๐ 2 views
Rapid identification and screening of pr
โ
Yajuan Chen; Dan Wall; David M. Lubman
๐
Article
๐
1998
๐
John Wiley and Sons
๐
English
โ 97 KB
๐ 2 views
Non-porous reversed phase (NPRP) high-performance liquid chromatography (HPLC) has been used as a rapid method to separate proteins from whole cell lysates of human erythroleukemia (HEL) cells. Using phosphate-buffered saline (PBS) as a lysis buffer to extract proteins from HEL cells, more than 100