✦ LIBER ✦

Partitioning of chicken egg white proteins in polyelectrolyte/salt aqueous two-phase systems composed of polyethyleneoxide–maleic acid copolymer and potassium phosphate

✍ Scribed by Toshio Kajiuchi; Yoshitsune Shin-Ya; Hidetada Nagai; Koichi Fujiwara

Publisher: Wiley (John Wiley & Sons)
Year: 2002
Tongue: English
Weight: 106 KB
Volume: 77
Category: Article
ISSN: 0268-2575
DOI: 10.1002/jctb.595

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✦ Synopsis

Abstract

Aqueous two‐phase systems were formed from solutions of a polyelectrolyte, polyethyleneoxide–maleic acid copolymer, and potassium phosphate. The properties of such aqueous two‐phase systems were highly dependent on pH. This was reflected in the partition behavior of three chicken egg white proteins: lysozyme, conalbumin and ovalbumin. Separability of these three proteins was improved by the use of the polyelectrolyte, in comparison with when uncharged polyethylene glycol (poly(1,2‐dihydroxyethane)) was used as a phase‐forming polymer.

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## Abstract Aqueous two‐phase partitioning is one of the excellent bioseparation systems for proteins and other biomolecules in water‐rich solutions containing polymers or salts. To improve the partition properties of model proteins, chicken egg white (CEW) proteins were chemically modified with po