RNA Isolation from Solid Tumor Tissue
โ Scribed by J. Kitlinska; J. Wojcierowski
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 272 KB
- Volume
- 228
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr
In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing t
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