๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Isolation of Intact DNA and RNA from Plant Tissues

โœ Scribed by R. Soni; J.A.H. Murray


Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
325 KB
Volume
218
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.


๐Ÿ“œ SIMILAR VOLUMES


Rapid isolation of total RNA from mammal
โœ Mark Emmett; Barbara Petrack ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 1007 KB

A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr

The isolation of high-molecular-weight D
โœ J. Hattori; S.G. Gottlob-McHugh; D.A. Johnson ๐Ÿ“‚ Article ๐Ÿ“… 1987 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 973 KB

A procedure to isolate high-molecular-weight DNA from plant materials has been devised. With this procedure, high-molecular-weight DNA suitable for Southern transfer experiments has been isolated from over 30 plant species including angiosperms (both dicots and monocots), a gymnosperm, members of ot

A micromethod for the isolation of total
โœ Kamran Tavangar; Andrew R. Hoffman; Fredric B. Kraemer ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 687 KB

We have developed a simple and rapid procedure for the isolation of total RNA from small amounts of adipose tissue. Using this method, it is possible to obtain quantitative recovery of RNA from less than 300 mg of adipose tissue, with an average yield of 70 micrograms of RNA per gram of adipose tiss

Isolation of DNA from plant cells by gel
โœ P.F. Lurquin; G. Tshitenge; G. Delaunoit; L. Ledoux ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 568 KB

A method is described which allows the obtention of total DNA from cells of Chlamydomonas reinhardi in 1 day. It is based on the gel filtration on agarose of crude lysates incubated for 1 hr with pronase and subsequently brought to 2 M NaCl in order to dissociate nucleoprotein complexes. The DNA ob

Rapid isolation of mouse DNA from cells
โœ William Meinke; David A. Goldstein; Mark R. Hall ๐Ÿ“‚ Article ๐Ÿ“… 1974 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 403 KB

A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapa