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Rapid isolation of mouse DNA from cells in tissue culture

✍ Scribed by William Meinke; David A. Goldstein; Mark R. Hall

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
403 KB
Volume
58
Category
Article
ISSN
0003-2697

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✦ Synopsis

A rapid method for the isolation of DNA from mouse cells grown in tissue culture is described. Cells are lysed in 0.24 M sodium phosphate, pH 6.8 buffer, containing 1% sodium dodecyl sulfate, 8 M urea, and 10m3 M ethylenediaminetetraacetic acid, and the crude lysate applied to a column of hydroxyapatite. RNA and proteins are removed from the column with 0.24 M sodium phosphate buffer containing 8 M urea while DNA is selectively eluted with 0.48 M sodium phosphate buffer. There is almost total recovery of cellular DNA from the column and the DNA is virtually free from proteins and RNA. This extraction procedure indicates that a mouse cell contains about 8 X 10d pg of DNA.

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