✦ LIBER ✦

Apparent trophoblast giant cell production in vitro by core cells isolated from cultured mouse inner cell masses

✍ Scribed by Wiley, Lynn M.

Publisher: John Wiley and Sons
Year: 1978
Tongue: English
Weight: 328 KB
Volume: 206
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1402060103

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✦ Synopsis

Abstract

Mouse ICM's that were isolated from blastocysts by immunosurgery and subsequently cultured for four to five days were tested for their ability to form trophoblast giant cells (TGC's) in vitro. The cores were isolated from such ICM's by a second immunosurgery and cultured for 7 to 14 days. After two days of culture a variable percentage (30%–90%) of the ICM cores produced outgrowths containing cells that resembled those of cultured ectoplacental cone. Then the outgrowths became amitotic and produced vacuolated cells that enlarged to dimensions of 200 × 375 μm with nuclei as large as 53 μm in diameter. The time course of their development from the ICM cores, their morphology and their nuclear diameters suggest that these large cells were TGC's.

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Trophoblast regeneration by inner cell masses isolated from cultured mouse embryos

✍ Spindle, Akiko I. 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 393 KB

## Abstract The developmental potential of the inner cell mass (ICM) of the cultured mouse embryo was determined by testing the ability of the ICM to regenerate trophoblast in vitro. ICM's isolated by immunosurgery from either single or chimeric embryos were able to regenerate trophoblast when they