Rat liver ribosomes and polyribosomes could be immobilized in agarose gels at 4°C and pH 7.6, using KC1 or NaCl molarities of 0.25 or higher. The binding could be effected in the presence of excess protein and/or detergents. Polysomes attached to endoplasmic membrane fragments did not bind to agaros
Reversible immobilization of enzymes to hydrophobic agarose gels
✍ Scribed by Karin Dahlgren Caldwell; Rolf Axén; Jerker Porath
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 330 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract Purine polyribonucleotides poly(A), poly(G), and poly(I) associate reversibly with agarose gels at high NaCl molarities over the pH range 6–10, at 20°−40°C. Pyrimidine polyribonucleotides poly (C) and poly(U) could not be immobilized in agarose gels under the above conditions. However,
Graphite Fibrilsm are hollow tubes (0.01 x 1-10 pm) consisting of concentric layers of graphite. Fibrils can be chemically modified to introduce surface functionalities such as carboxyl groups. Carboxylated fibrils were further functiondied to amino alkyl derivatives which were covalently linked to