Reversible and irreversible immobilization of enzymes on graphite fibrilsTM

✍ Scribed by Liwen Dong; Alan B. Fischer; Ming Lu; Mark T. Martin; David Moy; David Simpson

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 599 KB
• Volume: 9
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/(sici)1099-1352(199634/12)9:5/6<383::aid-jmr269>3.0.co;2-z

✦ Synopsis

Graphite Fibrilsm are hollow tubes (0.01 x 1-10 pm) consisting of concentric layers of graphite. Fibrils can be chemically modified to introduce surface functionalities such as carboxyl groups. Carboxylated fibrils were further functiondied to amino alkyl derivatives which were covalently linked to an enzyme, horseradish peroxidase (HRP). HRP fibrils showed substantial catalytic activity. Additionally, carboxyl fibrils were derivatized with specific inhibitors of the enzymes B-galactosidase @-Gal) and alkaline phosphatase (AP). Enzyme inhibitor-modified fibrils were used to specifically purify both B-Gal (using B-Gal inhibitor fibrils) and AP (using AP inhibitor 5brils) from mixtures of these two enzymes. These studies demonstrate the feasibility of using Graphite Fibrilsm as supports in biocatalysis and biospecific affinity chromatography.